Why, why, why? can I not keep cells on glass?!
I (anybody) can get them to stay on plastic, no problem. And they’re fibroblasts – bloody self-spot-welding limpets! Stick like shite to a blanket! But, trying again on glass coverslips (because the plastic supposed wonder-product for fluorescent microscopy coverslips turn out to be useless for bright-field), and I’m reminded how haphazard keeping them on glass is – or how inefficient I am. Despite all(?) precautions: washed in detergent; rinsed repeatedly in distilled water; immersed in acid-alcohol for one hour; air dry in the hood. Then coated overnight with fibronectin and gelatin; then plate the cells. And they seemingly attach fine; they look the same as on plastic. It’s when I come to fix them. They come off in a sheet (which I suppose suggests they hadn’t attached properly at all). Despite my carefully applied render, they’re rendered uninformative. Just (a) floating gossamer of gormless cells.
Sometimes it works; other times, like this morning, they detach so readily, that others in the corridor are commenting on that apparently Tourette’s-afflicted bloke in the lab who has just wasted another two days in an effort to nail a (probably pointless) figure.
Hang on, I’ve just thought of something…